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Mice were habituated in custom built behavior boxes equipped with a running wheel in a 12/12 light cycle for 10 days and fed ad libitum water, high fat diet (HFD, Research Diets D12492i) and regular chow (Labdiet Picolab 5053) in separate hoppers

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However, arm injections typically require assistance from another person or healthcare professional due to the difficulty in reaching the correct area safely

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Avoid carbonated beverages, which can trigger bloating and reflux when combined with semaglutide's delayed gastric emptying