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Acknowledgements We would like to express our sincere gratitude to Professor Zhentao Zhang from the Department of Neurology at Renmin Hospital, Wuhan University, for his invaluable guidance and support throughout this research
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For intracellular staining of phospho(p)-mTOR (Fisher Scientific 15549836) and p-S6 (eBioscience 15528216), cells were first stained for extracellular markers, fixed in 2% FA at RT, and stained for p-mTOR and p-S6 diluted 1:200 in saponin 5 g/mL
Nanomedicine 9, 12631273
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