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Corroborating these findings, immunoblot analysis of whole-kidney lysates demonstrated that UUO-induced upregulation of fibrotic (CCN2, FN1, COL1A1, and COL3A1) and ferroptotic (HMOX1) markers, alongside downregulation of GPX4, was more prominent in WT mice relative to KO littermates (Fig

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The PZQ LD 50 for NTS was determined after just 24 h exposure using both the Cell TiterGlo (27.1 2.27 M, Table 2) and the phenotypic analysis (42.9 0.83 M, Supplementary Movie 2), which is also used by others 54

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