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Interscapular brown adipose tissue was homogenized in Tris-ethylenediaminetetraacetic acid (EDTA) buffer (10 mM Tris and 1 mM EDTA, pH 7.4) with 1% protease inhibitor (Nacalai Tesque, Kyoto, Japan) on ice

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Retatrutide may help improve metabolic function by increasing energy use and supporting fat breakdown through its action on the glucagon receptor

Bulk RNAseq analysis Reads from RNAseq data were processed using the nf-core/RNAseq v3.17.0 pipeline and the GRCm39 genome assembly 55

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