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Blots were further incubated with anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (1:5000, 31460, Thermo Scientific) for 2 h at room temperature, and immunoreactive bands were detected using the enhanced chemiluminescence (ECL) method in a ChemiDoc XRS System (BioRad) with Image Lab software
& Dai, X
Free radicals and their impact on health and antioxidant defenses: a review
it triggers GH release without significantly elevating secondary hormones like cortisol, prolactin, or aldosterone
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doi: 10.3390/biom11081114 11 AshfaqS.AbramsonJ
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