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Procedures such as gastric bypass or sleeve gastrectomy have demonstrated significant long-term weight loss and improvement in obesity-related comorbidities, though patients require lifelong monitoring including thyroid function assessment, as malabsorption may affect levothyroxine requirements

Culture was scaled up into a 1 L flask and grown for a further 4 hours, and protein production induced with 0.5 mM IPTG for 4 hours

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Obesity and metabolic disorders have become major health concerns worldwide

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