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The day before the assay, Seahorse probe plates were prepared by adding 200 l distilled water, and calibration buffer was added to a 50 ml Falcon tube

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Here are a few simple tips: Use the lower belly or upper thigh as injection sites Always clean the skin first with an alcohol wipe Switch locations each time to avoid soreness Use a fresh, clean needle for every dose Dont inject into areas that already feel tender or irritated If youre not sure if youre doing it right, were happy to walk you through the process or even demo it for you in person