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For gene expression analysis, 1 g RNA was used for cDNA synthesis using a qScript cDNA Synthesis kit (Quanta Bio, 66196756)

Periodic acid-Schiff (PAS) staining was performed

Some users prefer longer cycles of 16 to 20 weeks followed by proportionally longer off periods

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Overall, although rapid progress has been achieved in this field in recent years, some crucial issues and challenges remain to be solved

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