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Storage requirements Temperature is the most critical storage factor

Rotating injection sites and allowing the solution to warm before injecting reduces occurrence

Purification of the digested samples was carried out via an ion exchange chromatography (Mono Q 5/50 GL column GE) with the running buffer: 10 mM Tris-HCl pH 7.8 and a size exclusion chromatography on a Superdex 200 increase column (GE) using a running buffer: 200 mM NaCl, 50 mM Tris-HCl, pH 7.8

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A 0.3mL (30 unit) syringe provides the finest graduations and easiest reading for these small amounts