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Statistical analysis All experiments were performed independently at least three times and the data were shown as mean and S.E.M

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2.10 Overexpression and knockdown of RRM2 gene in TPC-1 and IHH4 cells For upregulating RRM2 expression in cells, we obtained a full-length RRM2 sequence fragment using a PCR method and subsequentlycloned it into vector LV18, verified by DNA sequencing and transfected 293T cells (see Supplementary Materials for details)

Melanin is a natural pigment that improves the color of skin

Repeat to the desired dosage or as directed by a healthcare professional

Dehydration may cause new or worsening kidney problems, especially in people who already have kidney disease