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Replacement effects are summarized in the first paragraph followed by our data interpretation in the second paragraph: Regression and pattern analyses revealed sequential kinetics for all mutants with an apparent common intersection point at the x axis (Supplementary Fig

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The intracellular glutamate concentration is nearly 10-fold higher than the Km value, but intracellular cysteine concentrations approximate the apparent Km value [10] , indicating that cysteine may be a limiting substrate for GCL