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Measurement of the activity of senescence-associated -galactosidase (SA--gal) The activity of SA--gal in rats and patients liver tissues sections and cultured primary rat hepatocytes was determined using 5-bromo-4-chloro-3-indolyl P3-d-galactoside (X-gal), according to the manufacturer instruction (Senescence-associated -Galactosidase Staining Kit, Beyotime, C0602)

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Research has demonstrated involvement of the NO system, growth factor modulation (including VEGF, EGF), and FAK-paxillin signaling pathways