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Subsequently, the blots proceeded through a second round of immunodetection by agitating for 45 min at 50C in stripping buffer (2% SDS, 0.8% -mercaptoethanol (ME), and 62.5 mM Tris-HCl, pH 6.8) 32,56,62 followed by re-probing with HRP-labeled anti-HGF N -terminal domain mAb (1:2500 dilution in CanGetSignal solution 1) to monitor HGF -chain amount on the blots as loading controls

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doi: 10.1007/s00223-021-00911-7 91 CamilleriMVellaA

Similar content being viewed by others Introduction Neonatal abstinence syndrome (NAS) occurs in neonates after birth who have been exposed to opioids in utero

However, when the synaptic membrane is depolarized, Mg 2+ is removed, thereby allowing access to Ca 2+ ions

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