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The assay was characterized using the classic criteria of Richterich (sensitivity, specificity, precision, accuracy) and according to clinical analytical assay validation guidelines: Clinical and Laboratory Standards Institute (CLSI) guidelines for Immunoassays (I/LA23-A, I/LA21-A2, and EP24-A)
The study participants took 250 mg for the first two days and then were titrated up to 1000 mg
The slides were then quenched with peroxidase (methanol: PBS: H 2 O = 5:5:2) for 15 min at room temperature
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