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Quantitative reverse-transcription PCR (qRT-PCR) For qRT-PCR, the total RNA of testis was extracted by TRIzol reagent (Invitrogen, Carlsbad, USA)
Knowing how much bacteriostatic water to add to peptides prevents concentration errors that could lead to under or overdosing
All safety discussions apply strictly to research use
To verify this result, we assessed the selective sensitivity of VRK2-KO cells (CFPAC-1 cells and SW1990 cells) to APR-017 or LCS3 by employing colony formation assays
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