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The calibration curve for DA exhibited excellent linearity with an R 2 value of 0.99, indicating high sensitivity and reliable quantitative performance

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We analyzed the impact of acetylation on autoproteolysis at several lysine side chains located in the region we expect proteolytic processing of SnCE1 C256A to occur, i.e., Lys78, Lys94 and Lys98 in the N-terminal region, as well as AcK103, AcK106, AcK209 and AcK248 in the CE-clan protease domain