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Following confirmation of deep anesthesia, euthanasia was performed by exsanguination via cardiac puncture, in accordance with the American Veterinary Medical Association (AVMA) Guidelines for the Euthanasia of Animals (2020), which recognize exsanguination as an acceptable secondary method of euthanasia following adequate anesthesia

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Anesthesia induces neuronal cell death in the developing rat brain via the intrinsic and extrinsic apoptotic pathways

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Risks of Combining Sermorelin and MK-677 Stacking two GH secretagogues compounds several adverse effect categories