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The media was then replaced with glucose-free DMEM supplemented with 2% dialyzed FBS and 25 mM [U- 13 C]-glucose (Cambridge Isotope Laboratories, CLM-1396, 99% atom 13 C) for either 30 min or 2 h, or [U- 13 C]-palmitate (Cambridge Isotope Laboratories, CLM-409, 99% atom 13 C) for 24 h

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This method ensures maximum bioavailability and it allows for immediate utilization by the bodys cells

364 The metabolic reprogramming is characterized by shifting glucose metabolism to aerobic glycolysis, including enhanced cytosolic lactate fermentation and PPP and decreased OXPHOS

Provided as lyophilized powder with characteristic blue coloration

PMCID: PMC3448038