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This linear fragment contained the tag of choice (3x HA for COWP1-HA and HAP2-HA parasite lines) or loxP-flanked recodonised genetic regions and split Cre recombinase (DiCre) genes (inducible GR -KO line), as well as genes for neomycin resistance and nanoluciferase expression, flanked by 50 bp regions of homology to the cut site

This synthetic version is identical to the natural citrus form but easier to stabilize and use in formulations

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