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Dissolution in suitable solvent (e.g., water + acetonitrile) Reduction and alkylation (if disulfide bonds are present) Enzymatic digestion (optional for bottom-up sequencing approach) Key Insight: Clean and well-prepared samples significantly reduce noise and improve detection sensitivity

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Patients should aim to drink at least eight glasses of water a day, and more if they are exercising or losing weight quickly

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[43] The marketing tactics employed by Novo Nordisk are not only dangerous, but are arguably unethical