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GSH is synthesized from three constituent amino acids, namely, cysteine, glycine, and glutamic acid, with cysteine being a rate-limiting precursor

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However, some scholars have proposed that the effect of GLP-1 in the liver is indirectly mediated by multiple mechanisms, such as reducing fat absorption or inhibiting fat cells through acyl-CoA synthetase long chain family member 1 (ACSL1), CCAAT/enhancer binding protein (C/EBP), peroxisome proliferator-activated receptor- (PPAR), and sterol regulatory element binding protein-1c (SREBP-1c) pathways to achieve the purpose of reducing liver lipids [38]

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If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution