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Membranes were blocked with 5% non-fat milk and incubated with mouse anti-5mC (#61480, Active motif) or anti-5hmC (#39999, Active motif) followed by a horseradish peroxidase-conjugated antibody (Santa Cruz Biotechnology)

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The shedding usually stabilizes within a few weeks

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Visible peeling typically occurs days 3-7 after treatment

Our findings revealed that mice in the disease control group, exposed to rotenone, exhibited behavioral defects