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For comparison with leaching of molecular dye, similar PLGA nanoparticles were loaded with Nile red, a popular dye used for labeling PLGA and other polymers 50 , and found to leach 32.2 6.7% of initial load after only 6 hrs
Growth in 3D gels strongly correlates with tumorigenicity [25], and this property is used to distinguish tumorigenic from non-tumorigenic cells in vitro
Anal Biochem 1993
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