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To test whether this was due to insulin itself or to the drop in blood glucose that insulin causes, animals were clamped with high insulin while their glucose was either kept normal or made low

Each sample (8 l) was injected onto an Agilent Poroshell 120 EC-C18 (100 2.1 mm, 2.7 um) in a Shimadzu Nexera UHPLC system at a flowrate of 0.2 ml/min using a gradient elution created between buffer A (10 mM tributylamine aqueous solution adjusted pH to 4.95 with 15 mM acetic acid) and buffer B of methanol, from 10% to 40% (buffer B) in 30 min at room temperature

and compression at 50 N hardness on an NP-RD10 tablet press (Natoli, St Charles MO, USA)

Diabetologia 50:19001909 Marx N, Burgmaier M, Heinz P et al (2010) Glucagon-like peptide-1(137) inhibits chemokine-induced migration of human CD4-positive lymphocytes

US Patent WO 2014102692 A1: Method of treatment using lactobacillus fermentum me-3

These were never on Category 1, so they were never freely compoundable