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GAPDH and PKM2 are two major enzymes in glycolysis that regulated by sirtuins
Subsequently, the protein samples in equal volume were run on a 13%15% sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis, then moved to nitrocellulose membranes
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This is an irreversible biological change, unlike the temporary metabolic boost claims associated with Riboflavin-based products, which may not offer the same permanent results
