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This is perfect for bypassing the skin and entering the subcutaneous fat layer without hitting muscle
The four subcutaneous areas that work best are the lower abdomen, the outer thigh, the back of the upper arm, and the flank or love-handle area
At each time point (five minutes intervals), photoluminescence spectra was recorded using a FluoroMax 4 (Horiba, Jobin Yvon GmbH, Unterhaching, Germany) spectrophotometer working with a continuous 450 W xenon lamp at ex / em of: 365/605, 552/636 and 495/519 nm for PLGA encapsulating DDA-GSH-AuNCs, Nile red or fluorescein, respectively
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A p -value of less than 0.05 was considered to indicate significance