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(5) Cut the intestinal segment into small pieces of about 2-5 mm and transfer them to a 50 mL centrifuge tube containing 25 mL of pre-chilled PBS
Antibiotic use, probiotic supplementation, and dietary patterns all influence gut microbiome composition and therefore TMAO production
In experimental systems, this allows for more reliable analysis of the stimulation of pituitary somatotroph cells and pulsatile growth hormone release
doi:10.2147/IJWH.S340537
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