Cells were starved for nitrogen in a carbon free M9 medium for 3 h at 37 C, harvested by centrifugation and adjusted to the optical density of 3.0 in 20 mM TBS buffer (pH-7.2)
Reported side effects include skin rashes, abdominal pain (in rare instances), and temporary lightning in patches, which normalizes during treatment
Thus the dose, timing, and disease-specific inflammatory response are important pharmacological considerations with GSH treatment (Montero et al., 2023)
These early signs are encouraging but not universal
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