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Interestingly, changes in calcium homeostasis in the brain may contribute to the cognitive decline associated with normal aging and possibly to the development of neurodegenerative disorders (61, 62)

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The crude peptides were subsequently purified by reverse-phase chromatography on a C18 column with a linear gradient of water and acetonitrile containing 0.1% TFA, and their molecular masses were verified by liquid chromatographymass spectrometry (LC/MS)

Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)

Source: Xie et al., Signal Transduction and Targeted Therapy (2020)