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Cell pellet was resuspended in 65 l PBS and 65 l 2 SDS running buffer under mild ultrasound sonication (10 s, 10%, 5 cycle)

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These findings highlight that gut microbial alterations, beyond just TMAO elevation, may contribute to cerebrovascular disease progression (Yin et al., 2015)

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[MDPI] 41

The kit includes an injection pen, sterile needle tips, a durable carry case, and a pre-mixed BPC-157 cartridge, offering a ready-to-use solution