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Mice were housed in groups of six to eight in individually ventilated cages, with a 12 h light cycle (lights on 06:0018:00) and ad libitum access to standard chow (Altromin Spezialfutter, Lage, Germany) and water
These two approaches take advantage of the expanded dynamic range (2-fold FRET) that H188 exhibits in comparison to earlier FRET reporters that were used in microplate reader assays as readouts for cAMP signaling 31,34,66
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