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Positive control and negative control Positive control: Select the control strain based on the characteristics of the test sample (such as Staphylococcus aureus, Escherichia coli, etc.), with an inoculation amount of less than 100CFU

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Recordings were made at room temperature in artificial cerebrospinal fluid (aCSF) (pH 7.3, osmolarity 305 +/- 5 mOsm/kg) perfused at 2 mL/min containing 150 mM NaCl, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 10 mM glucose, 3 mM potassium chloride, 2 mM calcium chloride, and 1.3 mM magnesium chloride

doi: 10.1016/j.semcancer.2019.03.002 45 LiuJXiaXHuangP