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Recommended Testing Schedule Heres a suggested testing plan for B12 treatment: First test B12 levels before starting treatment Check again after the first dose (usually 1-3 months later) Test every 3-6 months while taking maintenance doses Test more often if you have trouble absorbing B12 or other health issues Interpreting B12 Levels During Treatment When looking at B12 levels, doctors must think about a few things

some clinicians may start at the lower end (0.2 mg) and adjust based on IGF-1 levels and clinical response

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While the etiology of NTD is multifactorial, some studies have found that blood concentrations of homocysteine or holo-transcobalamin to be lower in NTD-affected infants and mothers compared to non-affected controls (reviewed in 59)

Disinfect the work surface if appropriate for your laboratory environment

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