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The adaptor-ligated cDNA library was then PCR amplified and hybridized by SureSelect XT Mouse All Exon probes (Agilent Technologies, catalogue number 5190-4641)

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After deparaffinization and hydration, the sections were treated with heated citrate buffer (pH 6.0), 0.3% H 2 O 2, and blocked with 3% BCA successively, then incubated with primary antibody at 4 C overnight and biotinylated secondary antibody