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To minimize the time that cells are kept in MAS buffer, just before calibration finished, the cell media was removed, and the cells were washed once with MAS (1) buffer before addition of 180 l MAS (1) buffer

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The crude material was purified by Isolera flash column chromatography on silica via gradient elution, from 100% dichloromethane to 15% methanol in dichloromethane to produce CAM-S-CAM- d 15 TPP (9.1 mg, 27%) as a pale yellow fluffy/foamy solid

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