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All rats used in this study were kept at a humidity of 50%60% and a temperature of 25C, with food and water being freely accessible

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In this process the GSH gets converted to glutathione disulfide (GSSG)

Primary mouse peritoneal macrophages, mouse macrophage-like RAW 264.7 cells, and human monocytic THP-1 cells were transfected with GSTP encoding expression vector (pcDNA3.1-Flag-GSTP) or empty vector (pcDNA3.1), followed by stimulation of LPS (500 ng/ml) for 18 h, and the levels of HMGB1 in the culture medium were determined by ELISA

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