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Immunofluorescence For immunofluorescence experiments performed in U2OS cells, cells were seeded on 8-well chamber slides (Ibidi, 80826-90) with 40,000 cells per well, and transfected 24 h later and fixed 24 h after transfection with 4% PFA in PBS for 10 min
BMJ Open 13 , e063026 (2023)
10.3389/fimmu.2019.00155 59 LinC
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[ 9 , 10 ] However, despite decades of investigation, much of the available evidence comes from laboratory studies, animal models, or relatively small human trials
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