Following incubation with HRP- labeled secondary antibodies (1:5,000, Abcam, UK), signals were revealed via enhanced chemiluminescence (ECL) reagents (Millipore, USA) and captured with a ChemiDoc MP platform (Bio-Rad, USA)
It is accepted that an excessive burden of ROS may lead to abnormal cell growth, corresponding changes in intracellular homeostasis and damage to important components of in the cell when the antioxidant defense and/or DNA repair mechanisms cannot balance the excess oxidants [29]
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Its targeted mechanism of action also makes it a generally safe and well-tolerated peptide
The garlic ingredient diallyl sulfide inhibits cytochrome P450 2E1 dependent bioactivation of acrylamide to glycidamide
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