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The DNA of Gstp knockout mice was extracted and subjected to PCR by using MightyAmp Genotyping Kit (Takara, Ohtsu, Japan), which as shown in Figure S1C in Supplementary Material
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Sleep is equally relevant: testosterone is primarily produced during sleep, and research has found that even one week of sleeping five hours per night reduces testosterone levels by approximately 10 to 15% in young men, a reduction equivalent to ageing ten to fifteen years
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