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doi: 10.1016/j.freeradbiomed.2020.10.002 128 JarrottB.HeadR.PringleK

The mean fluorescence intensity of the ROS signal of cells in a 0.18 mm 2 field was calculated using ImageJ (version 1.51m9, National Institutes of Health, Bethesda, MD)

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I have heard of members using out of date ampoules, but not as out of date as yours