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H., Choi, S., Kim, D., Park, H

At day 20 (passage 3), epSPCs from G93A-SOD1 and B6SJL mice were cultured at a density of 10 5 cells in proliferative medium consisted of Dulbeccos modified essential medium (DMEM Thermo Fischer Scientific) supplemented with F12 (Thermo Fischer Scientific), 50 ng/ml epidermal growth factor (EGF

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By using Western blot, immunofluorescence and other techniques, the expression and distribution of tight junction proteins were analyzed, and the effect of IGF-1 LR3 on barrier integrity was studied

Blood samples were collected from participants on days 7, 14, and 21