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Ive had IV treatments in the past at other places and honestly never felt like they made much of a difference, so I wasnt expecting a miracle

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For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35