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Subsequently, the RAW264.7 cells were washed with PBS and stained by Ca 2+ Probe Fluo-4 AM for 20 min

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Verdict: Works, but impractical, expensive, and carries real risks

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The recent launch of the new Ombion Center for animal-free biomedical innovations is a demonstration of major investments by the Dutch government to build an infrastructure offering research, education, training, advice and support to enhance the acceptance and use of animal-free biomedical innovations (323)

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