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After additional 3-min incubation, 40 L of acetic acid was added, and 20 L of the reaction mixture was injected into a 150 4.6 mm Hypersil ODS column (Thermo Fisher Scientific, Waltham, MA, USA) equilibrated with 30 mmol/L ammonium nitrate and 40 mmol/L ammonium formate buffer, pH 3.6 (buffer A)

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