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Based on the assessment of alpha rarefaction, a threshold of 6,407 sequences/sample was established and used to normalize samples for uneven sequencing depth for the subsequent diversity analyses (42)
In addition, the framework for probiotics should include some well-defined beneficial symbiotic microorganisms, such as Akkermansia muciniphila and Faecalibacterium prausnitzii , along with other butyrate-producing bacteria such as Roseburia spp
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