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The first step is confirming that B12 is genuinely needed
Dihexa is an N-hexanoylated tripeptide derived from Angiotensin IV that acts through HGF/c-Met receptor activation
296 , jbc.RA120.014682 (2021)
Journal of vascular surgery
The binding of HGF to c-met was detected with biotinylated anti-HGF polyclonal Ab (1:500 dilution in the 0.1% BSA-TTBS for 3 h at 25C), TACS streptavidin-HRP conjugate (1:1600 dilution for 20 min), and TMB substrate solution
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