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Short-Term Sleep Deprivation Leads to Decreased Systemic Redox Metabolites and Altered Epigenetic Status. PLOS ONE , vol

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GSH biosynthesis occurs exclusively in the cytosol via a two-step ATP-dependent reaction catalysed sequentially by two enzymes: gamma-glutamylcysteine synthetase (GCL also referred to as gamma-glutamylcysteine ligase, comprising the catalytic GCLC subunit and regulatory GCLM subunit), which catalyses the rate-limiting first step of joining glutamate and cysteine

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This region shows an electron density high enough for constructing main-chain atoms (Fig 1A and 1C)