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If too little is added, the solution becomes more concentrated and harder to measure accurately

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10.1124/jpet.301.1.77 J

The 5-FU mouse model was selected because it is rapidly developed, cost-effective, and closely reflects the pathological features observed in human CIM, making it a widely used approach for evaluating therapeutic candidates (Huang et al

J Cell Biol 104(3):689696 Madri JA, Pratt BM, Tucker AM (1988) Phenotypic modulation of endothelial cells by transforming growth factor-beta depends upon the composition and organization of the extracellular matrix