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Vitamin C is a critical co-factor in collagen synthesis
While the Fe-S cluster is critical for activity, its role for the reaction mechanism remains unclear ( in vitro using glutathione disulfide (GSSG) or in vivo in response to ozone and paraquat, and inactivated upon reduction ( Four APK proteins exist in Arabidopsis, APK1, 2, and 4 locate in plastids whereas APK3 is in the cytosol (Mugford et al., 2009)
Comparing peak area ratios allows verification of approximate component ratios (though mass response factors differ between peptides, so precise ratio verification requires calibration curves)
The two protocols address complementary layers of cellular wellness and are often run on alternating weeks within a longevity-focused plan