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Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator
Mitochondrial size and density increases, fusion/fission dynamics are dysregulated, ETC efficiency decreases, and ROS production increases (De Vivo et al
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This makes Glutathione the most abundant endogenous, influential antioxidant to exist, supporting antioxidant activity to help regulate melanin pathways involved in discoloration
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